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2022 HSC Biology Exam Solutions

Want to see how you went in the 2022 Biology HSC exam? Read on!

The Matrix Biology team is thrilled to publish the 2022 HSC Biology Exam Solutions. These are the responses for the 2022 HSC Biology exam which can be viewed here.


2022 HSC Biology Exam Solutions

Keep in mind that these answers were not written under exam conditions and may be longer than required.


Section I: Multiple Choice

Question Answer Solution
1 B Vasodilation brings heat to the skin surface where it is lost to the air (usually with the assistance of sweating to produce evaporative cooling).
2 C This is an automatic internal response of the body.
3 A Antibodies are defensive proteins produced in response to an antigen.
4 B Pain, warmth, redness and swelling are all symptoms of inflammation.
5 B The data is split by categories, so a column graph should be used.
6 D The blastocyst (early embryo) implants into the endometrial lining of the uterus.
7 D The nucleus is discarded from a donor egg and the somatic cell nucleus is inserted.
8 B Muscle cells are diploid somatic cells. 40 chromosomes total means there are 20 pairs.
9 D People over 65 consistently experience a lower effectiveness than the younger age groups.
10 B The variable that was changed for each test was the flask.
11 A The purpose of the experiment was to demonstrate that cells arise from existing cells (in the air) and cannot spontaneously generate.
12 A Sugar is typically represented as a pentagon and phosphate is represented as a circle. Cytosine binds with guanine so Z cannot be guanine.
13 C Cytosine and guanine have changed to thymine and adenine, so this is a change in one base pair.
14 A Unaffected parents have affected offspring, which indicates it is recessive. Since the father in generation II is unaffected (XRY or Rr) but has passed a recessive allele to a daughter it must not be sex linked.
15 C Parent 1 is Rr and parent 2 is RR. Since red (R) is dominant both parents will be red.
16 B Based on previous questions, this is the safest answer even though C is also true!
17 B In metaphase 1 of meiosis, chromosomes line up in homologous pairs.
18 A A mutation has occurred in a germ cell that went through meiosis to become the eggs produced by individual 2 that were fertilised and became individuals 4 and 5. Genetic diseases are not caused by somatic mutations.
19 D Myopia causes the focal length to be short, so the focal point does not reach the retina at the back of the eye. A convex lens will make the focal length even shorter.
20 D The blood and dialysate flow past each other in opposite directions. The fresh incoming dialysate has a low concentration of urea. The incoming blood has a high concentration of urea.


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Section II: Extended Response

Question 21

(a) A pathogen can pass from person to person through direct transmission. This involves physical contact between individuals such coughing or sneezing directly onto someone or shaking hands.

(b) No cells: Virus OR prion

Nucleus present: protozoa

Nucleus absent: bacteria

Question 22

Since there are three phenotypes, and one (violet) is in between the other two (white and dark purple) this must be incomplete dominance. The violet offspring would be heterozygous and the others would be homozygous.

If E1= dark purple and E2 = white then E1E2 = violet

If two violet eggplants are crossed:

E1 E2
E1 E1E1 E1E2
E2 E1E2 E2E2

The phenotypic ratio of the offspring will be 25% dark purple 50% violet and 25% white (1:2:1).


Question 23

(a) Pollen is manually transferred from the male anther to the female stigma. This can be achieved by transferring pollen using a paintbrush or by removing the stamens and rubbing them on the stigma of the other flower.

(b) Artificial pollination can be used to introduce genes from a different population and thus increase genetic diversity in a population. However, if the same pollen is used many times, it will decrease genetic diversity.


Question 24


(b) From the age of 20, the prevalence of chromosomal abnormalities increases exponentially as the age of the mother increases.

(c) Trisomy is a type of chromosomal mutation where there is one chromosome added to a homologous pair. This occurs due to non-disjunction in meiosis where chromosomes fail to separate, resulting in a gamete with two copies of a chromosome instead of one. When the gamete is fertilised, there will be 3 copies of a specific chromosome instead of 2 (total chromosomes 2N + 1). An example is Trisomy 21 (Down syndrome) where the zygote ends up with three copies of chromosome 21.


Question 25

Asexual reproduction and sexual reproduction are both methods of producing offspring but in asexual reproduction there is only one parent and in sexual reproduction genetic information from two parents is combined to produce the offspring.

In asexual reproduction the parent undergoes mitosis (or binary fission) in order to produce offspring. An example for both animals and plants is budding, where the offspring grows on the body of the parent and then breaks away to become independent. The result of asexual reproduction is usually offspring that are genetically identical clones of the parent, resulting in no genetic variation. In rare cases, mutation could occur in the first cell of the forming offspring that results in genetic variation.

Sexual reproduction involves the fusing of male sperm and female egg to combine chromosomes from both parents. The formation of gametes through meiosis means that the gametes are genetically unique. It is then random as to which genetically unique egg and sperm meet during fertilisation.

Crossing over occurs in prophase I of meiosis and involves the swapping of chromosome segments between the maternal and paternal chromosomes of a homologous pair. This results in new combinations of alleles in the gametes. During metaphase I of meiosis, homologous pairs line up randomly on the metaphase plate, and it is random as to which maternal and paternal chromosomes are allocated to each daughter cell (random segregation). This results in unique combinations of chromosomes in the gametes.

As a result of these processes, sexual reproduction produces more genetic variation than asexual reproduction and it is still possible for mutation to occur during sexual reproduction further contributing to genetic variation.


Question 26

Note: there are many ways to answer this question!

1. Pure Methylglyoxal is extracted from the honey and diluted to 5 different concentrations: 100%, 75%, 50%, 25% and 10%.

2. 3 agar plates are each inoculated with a different bacterium using a disposable swab and labelled e.g., E. coli, S. aureus and S. pyogenes.

3. Using sterilised tweezers, 5 Paper circles are each dipped into one of the 5 Methylglyoxal concentrations and placed on each inoculated agar plate. One paper is not dipped as a control.

4. A control plate is set up with agar that has not been inoculated and no paper disks.

5. All agar plates are placed in an incubator at 37oC for 5 days.

6. After 5 days the diameter of bacterial inhibition around the paper is recorded for each plate.

7. Steps 2-6 should be repeated 5 times. For safety, inoculated agar plates must be immediately sealed with sticky tape and never opened again to avoid exposure to pathogens. At the end of the experiment used agar plates should be autoclaved or placed in two Ziplock bags before disposal.

2022 hsc biology solutions matrix 2a (1)

By Sommer36 – Own work, CC BY-SA 4.0,


Question 27

The incidence of cervical cancer in Australian women was declining before 1991, however the overall rate of decline increased between 1991 and 2002 after the national screening program was introduced. Detection of pre-cancerous cells on the cervix means infected cells can be removed before becoming cancerous, which led to a steep decline in cervical cancer incidence.

Since 2002 the incidence of cervical cancer has been consistent at around 7 per 100 000 women. The HPV vaccine for girls was introduced in 2007 and 2013 for boys, but since infection may not take place until many years after vaccination and it takes around 10 years for cervical cancer to develop after infection, long term data is needed to determine whether this vaccination campaign has successfully reduced incidence.


Question 28

(a) Model 2. (DNA replication is semi conservative, each copy has one original strand and one new strand.)

(b) The enzyme helicase unwinds the DNA by breaking the hydrogen bonds between complimentary bases. DNA polymerase enzymes run along each strand of the DNA adding complimentary nucleotides. The end result is two double stranded DNA molecules that are identical to the original strand.

(c) Eukaryotic DNA is linear, while prokaryotic DNA is always found in loops. Eukaryotic DNA is found as multiple chromosomes in a membrane-bound organelle called the nucleus while prokaryotes have a single large chromosome found in a region called the nucleoid. Prokaryotes also have short rings of DNA known as plasmids. A small amount of looped DNA is also found in the mitochondria and chloroplasts of eukaryotes.


Question 29

(a) Bt cotton was introduced in 2002 and cotton yield increased as the percentage of Bt cotton grown increased. The pesticide produced by Bt cotton would decrease damage to the plant caused by the bollworm pest and increase the amount of cotton harvested.

(b) The data indicates that Bt cotton produces an effective Bollworm insecticide. The data in the second graph shows that that the tonnes of bollworm insecticide were gradually reduced to almost 0 after Bt cotton became common after 2005. The reduction in the use of this pesticide means less cost and effort for the cotton farmer. This also means less insecticide leaching into the environment and poisoning non-target species, potentially reducing species diversity.

However, while cotton yield is still above 500 kg/ha compared in 2013 to above 300 kg/ha before Bt cotton was introduced, the total amount of insecticide used per year is also higher as hemipteran insecticide use increased to over 10 000 tonnes per year. So while Bt cotton is an effective method of control for Bollworm, it is not effective at controlling Hemipteran pests.



Question 30

Malaria is caused by a pathogenic protozoa (plasmodium) that is spread by indirect transmission via a mosquito vector. The female anopheles mosquito bites an infected host and ingests blood along with the plasmodium. When the mosquito bites an uninfected host, the pathogen enters the blood stream via the saliva of the mosquito.

Some regions of the world such as western Europe, north America and Japan have the mosquito vector (map 1) but no malaria (map 2). A possible reason for this is that malaria has been eliminated from the region so there are no infected individuals to pass on the disease/pathogen.

These regions are likely to have accessibility of medical facilities and pharmaceutical treatment so that there is early detection and treatment of infected individuals. Better quarantine of infected individuals ensures that they cannot pass on the pathogen. There is also likely to be better mosquito population control e.g., management of stagnant water (mosquito breeding areas), and prevention of bites through bed nets, long sleeves, and repellent.

Conversely some regions such as the West coast of south America, parts of northern and southern Africa, Arabian Peninsula and parts of China and Mongolia have malaria (map 2) even though the mosquito vector is not present (map 1). It is possible that infected individuals are moving into these regions from nearby infected regions due to high levels of travel and migration from these regions.



Question 31

(a) (i) While the number or women in each category was different, the sample size was large enough to confidently calculate mortality. Covering 29 health districts is helpful to avoid bias from environmental conditions that are only found in one health district. Non-smoker women with non-smoker husbands act as an important control group because this group demonstrates that 8.7% of lung cancer is not actually related to smoking in the household. 14 years is a significant time for a long-term study, but 20 years or longer would be an improvement as there is often a significant lag between smoke exposure and lung cancer. Overall, the study is valid and provides important data on the link between cigarette smoke and lung cancer mortality rate in women.

(ii) The study shows that 8.7% of lung cancer mortality in women is not related to smoking in the household. However, women who have a husband who smokes (passive smoking) have almost twice the lung cancer mortality rate of this control group. Women who smoke themselves have twice the rate of this passive smoking group and more than three times the lung cancer mortality rate of the control group. Therefore, the study shows that both smoking and passive smoking increases risk of death from lung cancer.

(b) (i) EGFR gene (DNA) -> RNA polymerase produces mRNA copy of the gene (transcription) -> mRNA copy leaves the nucleus and finds a ribosome -> ribosome reads codons of mRNA (translation) -> tRNA molecules with matching anticodons deliver amino acids -> ribosome joins amino acids to form polypeptide chain -> polypeptide is folded to form EGFR protein

(ii) Any mutation (permanent change) to the DNA sequence of the EGFR gene will also be copied onto the mRNA. Any change to the codons will also change the amino acids delivered by the tRNA that form the polypeptide. A change to the amino acids can change the bonds that form between amino acids that give the protein structure. A change to structure will affect function of the protein, particularly if the receptor or the active site of the enzyme changes shape and the protein cannot bind to the substrate. This will prevent the protein from doing its job to regulate cell division. Unregulated cell division can result in cancer.



Question 32

Normally a negative feedback loop exists where follicle stimulation hormone (FH) and luteinising hormone (LH) are released by the brain and received by the testes. Sertoli cells in the testes respond by increasing sperm production and producing inhibin, while Leydig cells respond by increasing testosterone production. The resulting production of testosterone and inhibin prevent further FSH and LH release until sperm or testosterone levels become low (and create a stimulus). If a male athlete continuously takes anabolic steroids (testosterone) this will result in an inhibition signal to be sent to the hypothalamus and the anterior pituitary. This will prevent the release of FSH and LH which will prevent sperm production in the testes. Over time, the athlete may find that they become infertile, and the size of the testes may decrease.


Question 33

Mutation (permanent change to DNA) is the only mechanism that creates new alleles in a population. It is likely that the allele that causes increased inflammation first appeared in the human population as a mutation.

Selection pressures can then increase or decrease the frequency of the allele in the population. It is possible that European, African and East Asian regions were exposed to the virus at some point in the past. Individuals carrying the allele were less likely to survive and pass on the allele and so the frequency reduced in these populations (<16 %). If the South Asian population was not exposed to the virus in the past, then the absence of this selection pressure would allow the allele to become more common in this population (60.3 %).

Genetic drift occurs in small, isolated populations. Random events (not selection pressures) cause the frequency of alleles in the population to change. It is possible that the South Asian population was isolated for some time and genetic drift allowed the allele to become more common in this population.

Gene flow occurs when new alleles are introduced to a population through migration and interbreeding from a different population. It is possible that the allele first appeared in the south Asian population and was introduced to the other populations (such as Europe 15.1 %) through gene flow.

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Now hiring 2022 HSC graduates to tutor English, Maths or Science! Roles are open at Strathfield, Chatswood, Epping and Hurstville.

Written by Matrix Science Team

The Matrix Science Team are teachers and tutors with a passion for Science and a dedication to seeing Matrix Students achieving their academic goals.


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